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In this conventional translation mechanism, the ribosome moves along the single-stranded mRNA and reads codon. But in the meantime, must first ribosome binding of mRNA 5 end of the cap structure. www.cusabio.com/ELISA_Kit-84594 il-6 elisa kit Sometimes ribosome binding without first 5 end of the cap structure of mRNA can begin to read the information, this mechanism does not depend cap structure is known as translation. Really nucleated cells undergo mitosis and apoptosis, there will be such a form of translation. In addition, many viruses are not dependent on the use of the translation of cap structure, so that the host ribosomal translation of viral transcripts priority. So far, people still do not understand the translation mechanism. Brian P. Wellensiek first author and his colleagues designed an in vitro screening strategy, to study the structure does not depend hat translation, identification of the mechanisms responsible for the translation initiation sequences, and verify the functionality of these sequences. Article explains senior author John Chaput, previous studies do not rely cap structure translation, mostly derived from viral RNA fragments used. "These RNA molecules can be folded to simulate some of the eukaryotic translation initiation factor," he said. Recently, there has also found in cells similar RNA molecule, but these short RNA sequences, its mode of action is different from the viral RNA. In order to identify the range of the protein food diet of the RNA, genomic researchers first cut into random fragments of approximately 200bp construct the entire human genome DNA library, and for the transcription of these sequences. They used the mRNA display technology, the success of the amino acid sequence of the transcription of mRNA binding fragment remains. On this basis, the researchers screened a translation reinforcing elements, and found that these elements gathered in non-coding regions of the genome. Subsequently, researchers will enhance the translation element, inserted into a vector with a hairpin structure. "If the ribosome 5 cap structure combined, will meet the hairpin structure and failure if the ribosome skip hairpin identify and translate the back of the sequence, it means that the sequence may be used as the endogenous ribosomal initiation site. "Chaput explained. Thus, the researchers further understand the translation of cap structure does not depend on a genome-wide mechanism for positioning the starting sequence. This study can help people further understand the underlying protein food diet of translation, but also for the field of biomedical research has brought important revelation. Because many viruses do not rely cap structure through translation, to hijack cellular translation mechanism to translate viral proteins priority.

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